CRISPR/Cas9 is a gene-editing tool that can manipulate gene expression in plants, humans, and animals. CRISPR, or Clustered Regularly Interspaced Short Palindromic Repeats, are short sections of bacterial DNA containing repetitive base sequences. CRISPR plays a crucial role in the immune response of bacteria against foreign DNA. When a bacterium detects viral DNA, it produces two strands of short RNA called guide RNA, which then go on to form a complex with an endonuclease enzyme called Cas9 (CRISPR-associated protein 9).
This complex targets and cuts out the viral DNA rendering the virus disabled. The Cas9 nuclease will not bind to the DNA if the target sequence is not followed by the Protospacer Adjacent Motif, or PAM, which helps the enzyme distinguish between the bacterial DNA and the viral DNA target. The CRISPR/Cas9 system then has the ability to store this viral data so that it will be able to recognize and eliminate future viral threats.
This process can be done in a cell’s nucleus, in stem cells, embryos, and even extracellularly in a test tube. CRISPR/Cas9 is able to edge out other gene-editing techniques like TALEN and Zinc finger nucleases because it is the only technique that is highly efficient and precise, extremely customizable, and can target multiple genes at once.
The versatility of the CRISPR/Cas9 system and its ability to locate and alter specific genes can yield advancements in drug discovery, basic medical research, agriculture, and even the possibility of preventing genetic diseases, heart disease, and blood conditions in humans. One example is the possibility of creating a Malaria-resistant Mosquito, in which scientists were able to alter multiple genes in a type of mosquito that allows it to become resistant to Plasmodium falciparum, the parasite that causes Malaria.
Scientists are also utilizing CRISPR/Cas9 to make advancements in the fight against HIV. In a study led by researchers at the University of Pittsburgh and the Lewis Katz School of Medicine at Temple University, the CRISPR/Cas9 system has shown promise in effectively locating and shutting down HIV-infected human immune cells in mice. The researchers look to take the next step toward human application by testing their findings in primates.
CRISPR/Cas9 also will play a multi-fold role in agriculture by making it possible to edit crops to make them more nutritious, better tasting, disease-resistant, and less susceptible to drought. The benefits of CRISPR/Cas9 gene editing are seemingly endless across multiple areas of study.
In this course, however, we are going to learn about the CRISPR/Cas9 with the example of the living modal of mice, because the mouse is the best modal to mimic the human-like genotype and the phenotype to work within the experiments and the research.
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What you’ll learn
- Discover the promise of CRISPR in genome engineering
- Distinguish 4 types of targeted nucleases available to precisely cut DNA
- Compare somatic cell and germline gene editing techniques
- Describe the basic mechanism that underlies the CRISPR/Cas9 genome engineering technology.
- Discuss biological uses for a dead Cas9 (dCas9) protein
- Evaluate CRISPR/Cas9 strategies for generating better mouse models
- Formulate a complete strategy for performing fundamental genetic engineering experiments
- Examine the process of making a precisely-defined, genetically modified mouse in six to eight months
- Analyze CRISPR/Cas9 complications, including off-target events and founder mosaicism
- And a lot more…
- Be desired to learn about CRISPR/Cas9, revolutionizing gene editing technology
- Have at least basic knowledge of English language,
- Have at least the basic knowledge of the genetics and the terms used in genetics.
Who this course is for:
- Students who are interested in new Technologies
- Students of Bioinformatics and Biotechnology
- Students who want to Polish their skills
- Students of Universities who are studying the Genetics, or the Bioinformatics